Detection of infectious hematopoietic necrosis virus in fish mucus.
نویسندگان
چکیده
منابع مشابه
Plaquing procedure for infectious hematopoietic necrosis virus.
A single overlay plaque assay was designed and evaluated for infectious hematopoietic necrosis virus. Epithelioma papillosum carpio cells were grown in normal atmosphere with tris(hydroxymethyl)aminomethane- or HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid)-buffered media. Plaques were larger and formed more quickly on 1- to 3-day-old cell monolayers than on older monolayers. Cell ...
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Infectious hematopoietic necrosis virus (IHNV) is a rhabdovirus that produces an acute, lethal infection in rainbow trout (Oncorhynchus mykiss). Fish that survive infection cease to produce detectable infectious virus at approximately 46 days after infection, yet there is evidence that survivor fish continue to harbor virus particles (B. S. Drolet, P. P. Chiou, J. Heidel, and J. C. Leong, J. Vi...
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Infectious hematopoietic necrosis (IHN)-a highly lethal infectious salmonid disease-has caused substantial economic losses in the European production of rainbow trout (Oncorhynchus mykiss) since the late 1980s. The causal agent of IHN is the IHN virus (IHNV) introduced from overseas. However, until today, its phylogeographic spread in Europe remains poorly understood. We therefore sought to elu...
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Infectious hematopoietic necrosis virus (IHNV, Rhabdoviridae), is the causative agent of infectious hematopoietic necrosis (IHN), a disease notifiable to the World Organisation for Animal Health, and various countries and trading areas (including the European Union). IHNV is an economically important pathogen causing clinical disease and mortalities in a wide variety of salmonid species, includ...
متن کاملDevelopment of a biotinylated DNA probe for detection and identification of infectious hematopoietic necrosis virus
A nonrad~oac t~ve DNA probe assay was developed to detect and ~ d e n t ~ f y infect~ous hernatopoiet~c necrosls virus (IHNV) uslng a dot blot format The probe a synthet~c DNA oligonuc leot~de labeled enzymatlcally w ~ t h biotln hybnd~zed spec~f~cal ly w ~ t h nucleocaps~d mRNA extracted from Infected cells early In the vlrus repl~cation cycle A r a p ~ d g u a n ~ d l n ~ u m th~ocyanate base...
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ژورنال
عنوان ژورنال: Fish Pathology
سال: 1989
ISSN: 0388-788X,1881-7335
DOI: 10.3147/jsfp.24.197